Hepatitis D virus IgM ELISA Kit
Mataupu Faavae
O lenei pusa e iloa ai le hepatitis D virus IgM antibody (HDV-IgM) i le toto o le tagata poʻo le plasma samples, polystyrene microwell strips o loʻo faʻapipiʻiina muamua i antibodies e faʻasino atu i polotini M immunoglobulin tagata (anti-μ filifili).A maeʻa ona faʻapipiʻi muamua faʻataʻitaʻiga o le toto poʻo le plasma e suʻesuʻe, o le IgM antibodies i totonu o le faʻataʻitaʻiga e mafai ona puʻeina, ma isi vaega e leʻi fusia (e aofia ai faʻamaʻi faʻapitoa IgG) o le a aveesea e ala i le fufuluina.I le laasaga lona lua, o le HRP (horseradish peroxidase)-conjugated antigens o le a faʻapitoa faʻatasi ma HDV IgM antibodies.A uma ona fufulu e aveese le HRP-conjugate e le'i fusifusia, ona fa'aopoopoina lea o vaifofo chromogen i totonu o vaieli.I le i ai o le (anti-μ) - (HDV-IgM) - (HDV Ag-HRP) immunocomplex, ina ua uma ona fufulu le ipu, sa faaopoopo le TMB substrate mo le atinaʻeina o lanu, ma o le HRP e fesoʻotaʻi atu i le faʻalavelave faʻalavelave e faʻafefe ai le tali a le tagata faʻatupu lanu i. fa'atupu le mea lanumoana, fa'aopoopo le 50μl o le Taofi Fofo, ma liliu samasama.O le i ai o le faʻaogaina o le HDV-IgM antibody i totonu o le faʻataʻitaʻiga na fuafuaina e se microplate faitau.
Oloa Fa'ailoga
Ma'ale'ale maualuga, fa'apitoa ma mautu
Fa'amatalaga o oloa
Mataupu Faavae | Ole su'esu'ega ole immunosorbent ole enzyme |
Ituaiga | Auala pu'e |
Tusi Faamaonia | NMPA |
Fa'ata'ita'iga | Sua tagata / plasma |
Fa'amatalaga | 48T / 96T |
Teuga vevela | 2-8 ℃ |
Ola fa'asa | 12 masina |
Fa'amatalaga Fa'atonu
Igoa o oloa | afifi | Fa'ata'ita'iga |
Hepatitis D virus IgM ELISA Kit | 48T / 96T | Sua tagata / plasma |