Su'esu'ega ole HFRS – Fiva Hemorrhagic with Renal Syndrome

Talaaga

O le siama o le Hantaan (HV) o le fa'ama'i muamua lea e nafa ma le Fiva Fa'ama'i ma Renal Syndrome (HFRS). O le HFRS ose fa'ama'i fa'ama'i fa'ama'i fa'ama'i fa'ama'i fa'asalalauina fa'alelalolagi e fa'aalia i le fiva, toto toto, ma le fa'aletonu o fatu. O le faʻamaʻi o loʻo i ai se faʻalavelave faʻafuaseʻi, faʻavavevave le alualu i luma, ma le maualuga o le oti, e faʻaalia ai se lamatiaga tele i le soifua maloloina lautele. Rodents (e pei o Apodemus agrarius ma Rattus norvegicus) o faatanoa autu ma ves o HV. O le fe'avea'i i tagata e masani lava e ala ile aerosolized excreta (mimi, feces, saliva), fa'afeso'ota'i tu'usa'o, po'o u ve'a. HFRS e mafai ona tupu i le tausaga atoa, ma o le faitau aofa'i lautele e faigofie. E tusa ai ma fuainumera WHO, 32 atunuu i le lalolagi atoa ua lipotia mai HV, aemaise lava le maualuga o le salalau i Asia i Sasaʻe, Europa, ma le Balkans.

Fa'ailoga Antibody Pe'a Fa'ama'i HV

A mae'a fa'ama'i pipisi o le HV, e fa'atupuina e le tagata fa'ama'i fa'ama'i fa'apitoa, aemaise lava HV-IgM ma le HV-IgG.

● HV-IgM antibodies: Auauna e fai ma faailoga serological o le vave faʻamaʻi, e masani ona aliali mai i totonu o aso talu ona amata faʻailoga, ma e taua tele mo suʻesuʻega faʻamaʻi.

● HV-IgG antibodies: E aliaʻe mulimuli ane ma e mafai ona tumau pea mo le olaga, e faʻaalia ai faʻamaʻi ua mavae poʻo le toe malosi. O le fa'a fa pe sili atu le fa'atupuina o le HV-IgG antibody titer i le va o fa'ata'ita'iga o le toto ma'i ma fa'amālōlō e fa'ailoa fo'i mo fa'ama'i pipisi.

Auala masani ole Su'esu'ega HV

O metotia su'esu'e o lo'o i ai nei mo le su'eina o le HV e aofia ai le fa'aesea o siama, PCR, serological ELISA, ma colloidal gold immunoassays.

● Fa'aleaganu'u Virus ma le PCR e ofoina atu fa'apitoa fa'apitoa ae e alu ai le taimi, mana'omia fa'atekinisi, ma mana'omia fale su'esu'e maualuluga, fa'atapula'aina lo latou fa'aogaina lautele.

● Micro-immunofluorescence (MIF) e ofoina atu le saʻo lelei ae manaʻomia se microscope fluorescence ma faʻamatalaga faʻapitoa, faʻatapulaʻaina le faʻaogaina masani.

● ELISA ma colloidal gold assays e faʻaaogaina lautele i nofoaga faʻapitoa ona o lo latou faigofie, saoasaoa, maualuga maaleale ma faʻapitoa, ma faigofie o le aoina o faʻataʻitaʻiga (serum / plasma).

Galuega Fa'atino

Fa'ailoga o le HV-IgM/IgG (ELISA) a Beier Bio

● Ituaiga Faʻataʻitaʻiga: Serum, plasma

● Sample Dilution: IgM ma le IgG su'ega e fa'aaoga muamua le fa'ata'ita'iga lelei ma le fa'afefiloi 1:11 (100µl sample diluent + 10μl fa'ata'ita'iga), fa'afaigofie le galuega ma fa'aitiitia le mamafa o galuega a le tagata faigaluega.

● Reagent Sauni-e-Fa'aoga: Ua saunia uma mea fa'afoliga se'i vagana ai le pa'u fufulu (20x fa'aoga). Fa'ailoga lanu mo le faigofie ona iloa

● Faiga Fa'a'au: 30 min / 30 min / 15 min; fa'atotonu atoatoa

● Su'esu'ega Galu umi: 450 nm ma le 630 nm fa'asinomaga

● Faʻailoga Faʻapipiʻi: 96 poʻo le 48 vaieli malepelepe, taʻitasi ma faʻamaumauga o oloa lolomi mo le suʻeina ma le faigofie.

Fa'ailoga o le HV-IgM/IgG (Colloidal Gold) a le Beier Bio

● Ituaiga Faʻataʻitaʻiga: Serum

● Taimi Su'esu'e: I'uga i totonu ole 15 minute; leai ni meafaigaluega fa'aopoopo e mana'omia; lelei mo suʻesuʻega vave i falemaʻi i fafo, faʻalavelave faʻafuaseʻi, ma faʻasalalau tagata maʻi

● Faiga: Fa'aopoopo le 10µl fa'ata'ita'iga i le pepa fa'ata'ita'i fa'ata'ita'iga lelei e fa'aaoga ai se pa'u; fa'amatala iuga i totonu ole 15–20 minute

Fa'ata'ita'iga Fa'afoma'i o le HV-IgM (ELISA), HV-IgG (ELISA), ma le HV-IgM/IgG (Colloidal Auro)