SARS-COV-2 Aofa'iga Ab Pusa Su'ega (Elisa)

O le SARS-CoV-2 Total Ab Test Kit (ELISA) e faʻavae ile immunoenzymatic assay mo le faʻamautinoaina ole SARS-CoV-2 antibodies ile sua o le tagata, plasma (EDTA, Heparin poʻo le sodium citrate).O vaieli micro plate e pei o se vaega mautu ua ufiufi i le recombinant SARS-CoV-2 recombinant Receptor Binding Domain protein.I le laasaga muamua o le incubation e fetaui ma antibodies faapitoa (SARS-CoV-2-IgG-Ab & nisi IgM-Ab) o loʻo iai i faʻataʻitaʻiga maʻi e fusifusia i antigens i le vaega mautu.I le faaiuga o le incubation e fufulu ese vaega e le fusia.Mo le laʻasaga lona lua o le faʻamaʻiina o le SARS-CoV-2 recombinant Receptor Binding Domain protein conjugate (SARS-CoV-2 recombinant Receptor Binding Domain protein peroxidase conjugate) ua faʻaopoopoina lea e fusia faapitoa i le SARS-CoV-2 antibodies (e aofia ai le IgG ma le IgM) e mafua ai. le faʻavaeina o immunocomplexes masani.A mae'a le la'asaga lona lua o le fufulu e aveese ai le tele o mea fa'apipi'i, ona fa'aopoopoina lea o le TMB/Substrate (step3).O le lanu lanumoana e tupu a'e e suia i le samasama pe a uma ona taofi le tali ile fofo taofi.O le mitiia o calibrators ma faʻataʻitaʻiga e fuafua i le faʻaaogaina o le ELISA micro plate reader.O fa'ai'uga mo fa'ata'ita'iga o tagata ma'i e maua ile fa'atusatusaga ile tau tipi.